In Vivo Pharmacology / Pharmacodynamics

Adgyl Lifesciences offers comprehensive pharmacodynamic and efficacy studies in several therapeutic areas in GLP and non-GLP for drug discovery needs and regulatory submissions. The studies are well supported by requisite histopathology, immunohistochemistry, clinical biochemistry, biomarker (qPCR, cytokines by FACS/ELISA, Western Blot, immune-phenotyping using FACS). The GLP Bioanalysis and Formulation analysis are also performed.

Adgyl Lifescience scientists possess 20 plus years of in vivo pharmacology drug discovery experience to conduct efficacy studies for multiple therapeutic areas, in-house validated 100 plus models on board, rich surgical skills and model development skills. New model may be developed as per sponsors needs. Validated models as presented as follows:

Oncology- Syngeneic and Xenograft mouse models using SCID/NSG/NOD mouse strains

  • Syngeneic animal models
    • Lung cancer model in C57BL/6J mice
    • Breast cancer model in BALB/c mice
    • Colon Cancer Mouse Model in BALB/c mice
    • Fibrosarcoma in BALB/c mice
  • Xenograft models
    • Adgyl offers heterotopic (subcutaneous) xenograft models in immuno-deficient mice using,
      • Cell lines provided by the client
      • > 20 cell lines available with Adgyl
      • Procured from ATCC on behalf of the client
    • Following are the immuno-deficient mice available for xenograft
      • BALB/c Nude or B6 Nude (T cell deficient)
      • NOD Scid (T and B cell deficient)
      • NOG or NSG mice (T, B and NK cell deficient)

Inflammation and Autoimmune Disease Multiple animal models for acute inflammation, respiratory, joint and skin inflammation, inflammatory bowel disease, SLE and multiple sclerosis

  • Acute inflammation:
    • Carrageenan induced rat paw edema
    • LPS induced endotoxemia
    • Air-pouch model
    • Arachidonic acid induced ear edema
  • Respiratory inflammation:
    • Bleomycin induced pulmonary fibrosis in C57BL/6J mice
    • Ovalbumin (OVA) induced allergic asthma & Rhinitis in BALB/c mice
    • LPS induced acute lung inflammation (Neutrophilia) in Wistar rats
  • Inflammatory bowel diseases:
    • DSS induced ulcerative colitis model in mice
    • TNBS (Trinitrobenzenesulfonic acid) induced Crohn?s disease model in rats
    • Oxazolone induced colitis mice model
  • Joint inflammation:
    • Collagen Type-II induced arthritis (CIA) in mice
    • Adjuvant induced arthritis (AIA)
    • Collagen antibody induced arthritis (CAIA)
  • Skin inflammation:
    • Imiquimod-induced psoriasis in BALB/c mice
    • TPA (2-O-tetradecanoylphorbol-13-acetate) Induced atopic dermatitis in Mice
    • Oxazolone Induced Contact Dermatitis in Mice
    • Chloroquine induced itch BALB/c mice model
  • Systemic Lupus Erythematosus
    • MRL/lpr mouse model of SLE
    • NZBWF1/J mouse model of SLE
  • Multiple Sclerosis (also offered under GLP)
    • MOG35-55-induced EAE in C57BL/6 mice
    • PLP139-151 Induced EAE Model in SJL/J Mice Model

Fibrosis models for lung fibrosis, liver fibrosis/NASH and kidney fibrosis

  • Liver Fibrosis/NASH
    • CCl4-induced rat model of liver fibrosis
    • CCl4-induced mouse model of liver fibrosis
    • MCD diet induced liver fibrosis in mice
  • Kidney fibrosis
    • Unilateral Ureteral Obstruction (UUO) model in mice
    • Folic acid induced kidney fibrosis in mice Lung Fibrosis
    • IPF (Idiopathic pulmonary fibrosis): Bleomycin induced lung fibrosis

The models are supported by strong expertise in ex vivo assays, histopathology, and PD marker studies

Hemorrhoid Model in Rat

  • Croton Oil-Induced Hemorrhoid Model in Rat

CNS Disease – Animal models for studies in Parkinsons Disease, depression, cognition, cerebral ischemia, behavior and motor coordination, epilepsy, blood-brain barrier integrity, etc.

  • Anti- Parkinsons:
  • Primary & Secondary Models
    • Haloperidol induced catalepsy in rat model
    • 6-OHDA induced circling behavior in nigrostriatal lesioned rats (acute and chronic)
    • Levodopa- induced dyskinesia and rotational behavior in hemiparkinsonian rats (acute and chronic)
    • MPTP-Induced Parkinsons Disease Model in C57BL/6J Mice
    • Tremorine and Oxtremorine antagonism/induced tremors/ataxia/spasticity/ salivation/lacrimation/hypothermia
  • Anti-Depressants:
    • Tail suspension test in mice
    • Force swim test / Despair swim test in rats/mice
    • Apo-morphine-induced hypothermia in mice
    • Novelty Suppressed Feeding (NSF) Test in rats and mice
  • Cognition:
    • Object recognition test
    • Behavior and Motor Coordination
    • Rotarod Method
    • Locomotor activity in open field test
    • Morris water Maze
    • Y-Maze and T-Maze test
    • Marble burying test
  • Safety Pharmacology Core Battery
    • Irwins Battery test in mice and rat
  • Anti-Epileptic Activity
    • Pentylenetetrazole (PTZ) induced Convulsions/Seizures
    • Blood-brain barrier (BBB) integrity
    • Acute effect of LPS using Evans blue
  • Anti-Anxiety activity
    • Staircase test
    • Elevated Maze
    • Resident-Intruder/Isolation Aggression test

Pain – Multiple animal models for acute, chronic neuropathic, inflammatory and post-surgical pain

  • Acute Pain models
    • Acetic acid induced writhing
    • Hot plate model (central) in mice and rat
    • Post surgery acute pain
    • LPS-induced inflammatory pain
    • Mild Thermal Injury Model
    • Carrageenan/Capsaicin induced thermal/mechanical hyperalgesia
    • Tail flick/Tail immersion test or other radiant heat methods
  • Chronic: Neuropathic pain model
    • Spinal nerve ligation (SNL) model of neuropathic pain
    • Sciatic nerve ligation (Partial) in rat model
    • CFA induced model of inflammatory pain
    • Chemotherapy (Cisplatin)-induced peripheral neuropathic pain rat model
    • Formalin-induced pain (persistent pain model)
    • Buprenorphine-induced tolerance/withdrawal in Cisplatin-induced neuropathic pain model
    • Mono-iodoacetate induced osteoarthritis

Metabolic Disease – Multiple animal models for the assessment of in vivo metabolic profile, in vivo cardiovascular profile and ex vivo studies

Gastrointestinal Disease – Interaction studies of pharmacological modulators using intestinal and colonic explants and sections, isolated fundus strips; multiple animal models including DSS-induced ulcerative colitis, TNBS-induced colitis, GI motility studies, peptic ulcers, etc.

  • Gastric Ulcers
    • Indomethacin induced ulcer model
      • Ethanol induced ulcer model in rats
      • Pylorus Ligation Induced Ulcer Model in rats
      • Cold Restraint stress Induced Ulcer (CRU) Model in rats
  • Gastric Motility
    • Gastric emptying time

Ocular Pharmacology

  • Atropine induced Dry eye Rabbit model
  • Alkali induced corneal injury & assessment of corneal wound regeneration in Rabbits
  • Uveitis in Rabbits Eye
  • Glaucoma in rats