Discovery Biology – In Vitro Biology

Molecular and Cell Biology:

  • Flow cytometry and FACS
  • Immunological techniques
  • Gene expression
  • Cloning, over expression and stable cell line generation
  • Protein purification and characterization

In Vitro Assays:

The 3 R principles of animal welfare (Replace, Reduce and Refine) are adopted and practiced by the organization, wherever possible by using animal alternatives. All these tests are offered in accordance with the European Centre for the Validation of Alternative Methods (ECVAM).
In vitro tests are performed with isolated tissues, organs or part cells instead of a whole organism, which can relate the human body tissue and be adequate to conclude on an information requirement.

  • Enzyme assays: Inhibition, activation, kinetic analyses
  • Functional assays: Cell signaling (Gs, Gi, Gq, GTPs), substrate uptake, glycolysis, glycogenesis, immunofluorescence-based measurements of cellular events, mechanism of action and PD marker studies
  • Ligand binding assays
  • Multiple detection modes: Radiometric, absorbance, luminescence etc.
  • In Vitro Skin Absorption: Franz Cell Diffusion Assay