Our team has outstanding experience and capability to develop fit for purpose ADME and In Vivo rodent and non-rodent pharmacokinetic studies to scientifically support client needs. We have expertise in developing quantitative LC-MS/MS method for complex biomarkers in bio-fluids and tissue samples.
Physiochemical Properties
- Kinetic
- Thermodynamic
- Biorelevant media (FaSSIF and FeSSIF)
Lipophilicity (Log D and Log P):
- Octanol-water/phosphate buffer partitioning
- Simulated Gastro Intestinal fluids (SGF, SIF)
- Biorelevant media (FaSSIF and FeSSIF)
- Aqueous buffers at different pH
- Plasma and blood stability
- Dosing formulation
- Metabolic stability/ intrinsic clearance
- Liver and intestinal microsomes
- S9 fractions
- Hepatocytes
- Direct glucuronidation in liver microsomes using UDPGA
- Reactive metabolites identification using GSH trapping assay
- Metabolite finger printing analysis in liver microsomes
- Metabolism based drug-drug Interactions
- CYP inhibition (single point, IC50) using human liver microsomes (HLM)
- Time dependent CYP inhibition in HLM (IC50 shift, Ki and Kinact)
- CYP reaction phenotyping (purified rCYPs/microsomes)
- CYP induction in plateable human hepatocytes
- 1A2, 2B6 and 3A4 enzyme activity
- mRNA analysis
- Cytotoxicity
- Intrinsic permeability-PAMPA
- Mono and bi-directional transport: CaCo-2 and MDCK cells
- In presence and absence of efflux drug transport inhibitors (P-gp, BCRP and MRP)
- Definitive plasma protein binding
- Microsomal protein binding
- Brain tissue binding
- Blood-to-plasma concentration ratio (RBC partitioning)
- HepG2 cell line
- Hepatocytes